Loading Miescher Nuclein into Tobacco Mosaic Virus

Letter
 Pestle-grind 20g of infested tobacco leaf. Add dissolved barnacle nuclein to final volume 100 mL. Stir to form slurry. Filter slurry through Chamberlain filter-candle into sealed sterile Erlenmeyer flask Co-incubate nuclein and virus for upwards of three days. Punch 1cm rounds of seaweed. Uncap Erlenmeyer only briefly to drop rounds into the slurry. Incubate 24 more hours. Withdraw seaweed rounds and place in filtered seawater broth on sterile petri dish. Culture 1-2 weeks. 

Observations: Resultant seaweed rounds needed only three days to grow before exceeding their container space. Untreated controls still remain growing at normal rate.

Victor F. Stein