Isolation of Miescher Nuclein from Barnacle

Letter
 Mechanically disrupt whole barnacle samples with pestle and mortar to fine powder Dissolve 5g in 95mL of preservation buffer: 1% sodium dodecyl sulfate, 50mM ethylenediaminetetraacetate, pH to 7.7 w/NaOH Extract organics with 1:1 phenol-chloroform mixture in 50mL aliquots Invert 50mL aliquots 10 times, allow to sit overnight Pipet aqueous phase to new centralized container  Victor F. Stein